Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/24/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Lane 1 – Ladder Lane 2 – Sample 1 Lane 3 – ENTRY SAMPLE Lane 4 – Sample 3 Lane 5 – Drosophila Positive Control Lane 6 – Drosophila Negative Control Lane 7 – Wolbachia DNA Positive Lane 8 – Water
|
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No visual band on gel electrophoresis result. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Phymata americana
WRITNA-12-COLEOPTERA
Harmonia axyridis
GRE-LCH Coleoptera
Apis mellifera