Prenolepis imparis – 2C2

April 8, 2025

Sample information
Picture
Photos by: Tyler C.
Location
Collection date 02/24/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Insecta Hymenoptera Formicidae Prenolepis Prenolepis imparis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane 1 – Ladder

Lane 2 – Sample 1

Lane 3 – ENTRY SAMPLE

Lane 4 – Sample 3

Lane 5 – Drosophila Positive Control

Lane 6 – Drosophila Negative Control

Lane 7 – Wolbachia DNA Positive

Lane 8 – Water

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No visual band on gel electrophoresis result.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
>2C2 AATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTA ATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTT TTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTC AATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTA ATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCT ATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTAT TTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACC TTAATACTTCCTTTTTTGACCCCTCAGGAGGAGGAGACCCTGTTCTTTATCAACATTTATTCTGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Prenolepis imparis was found to be negative for Wolbachia.
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