Prenolepis imparis – 2C1

April 8, 2025

Sample information
Picture
Photos by: Aravind N.
Location
Collection date 03/11/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Prenolepis Prenolepis imparis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane 1: Ladder

Lane 2: Sample 1 (C1)

Lane 3: Sample 2 (C2)

Lane 4: Sample 3(C3)

Lane 5: + Drosophila Control

Lane 6: – Drosophila Control

Lane 7: Wolbachia DNA Positive Control

Lane 8: H20 Control

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Gel Results: The band indicating Wolbachia presence was faint in reference to the controls, but it was nevertheless present.

Sanger Sequencing: The blast results revealed a ~99.02 – 99.26 Wolbachia percent identity in certain sections [NCBI Blast].
Wolbachia 16S sequence Download FASTA    Download AB1
>Prenolepis_imparis2C1_WOLBACHIA-WSPECF (390 bp) GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>Prenolepis_imparis2C1-CO1F (637) CTGAGCTGGAATAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTAGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACCTTAATACTTCCTTTTTTGATCCCTCAGGAGGAGGAGACCCTGTTCTTTATCAACATTTATTCTGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Prenolepis imparis was found to be postive for Wolbachia.
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