Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Prenolepis Prenolepis imparis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Lane 1: Ladder Lane 2: Sample 1 (C1) Lane 3: Sample 2 (C2) Lane 4: Sample 3(C3) Lane 5: + Drosophila Control Lane 6: – Drosophila Control Lane 7: Wolbachia DNA Positive Control Lane 8: H20 Control |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The band indicating Wolbachia was faint in reference to the controls although the visible band indicated Wolbachia presence. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Prenolepis imparis was found to be postive for Wolbachia. |
Formica Pallidefulva
Hylephila Phyleus
Thomisus onustus
Halictus Ligatus
WRI-JAL-18 Order Hymenoptera