Sample information
Picture
Location
Collection date	03/11/2025
Captive / Cultivated?	Wild-caught
Group	Thomas Jefferson High School for Science and Technology
Observations	In stream when caught.
Putative identification	Arthropoda Hexapoda Insecta Hemiptera Gerridae Aquarius Aquarius remigis
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	Controls worked, and band showed up with very strong results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Aquarius remigis was found to be postive for Wolbachia.