Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | In stream when caught. |
| Putative identification | Arthropoda Insecta Hemiptera Gerridae Aquarius Aquarius remigis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level |
Controls worked, and band showed up with very strong results. The sequencing results for CO1 show likely contamination or other errors in the process, however the Wolbachia sequencing shows a strong result. |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence | Download FASTA
|
| Summary | The Aquarius remigis was found to be postive for Wolbachia. |
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Wolbachia data
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