Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 03/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | Caught in the wild during TJHSST stream field trip near Indian Run on March 11 2025 |
| Putative identification | Arthropoda Malacostraca Isopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Chopped roly poly in half and extracted DNA from front half of organism (including entire head and eyes and a fraction of its abdomen) |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Wolbachia testing was done by a professional and not me |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
TGCTTGAGCAGGGGCTGTTGGAACAGCCCTTAGGATAATTATTCGAACTGAGTTAGGAAACCCTGGGAGGTTAATTGGAGACGATCAGATTTATAATGTGATTGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGCTTTGGTAATTGATTATACCTTTAATATTAGGGGCCCCTGATATAGCTTTTCCGCGTATAAATAATATAAGATTTTGGCTTCTTCCCCCATCACTAATTTTGTTATTAAGAAGAGGTTTAGTTGAAAGAGGTGTTGGGACTGGCTGGACTGTCTACCCACCTTTGGCAAGTGGAATTGCCCATAGAGGGGCTTCAGTAGATTTAGGGATTTTTTCCCTTCATTTGGCTGGAGCTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGAGCAGAGGGGATAAGGATAGATCGTGTCCCTCTATTTGTTTGGTCAGTGTTAATTACAGCAATTCTCTTGTTACTTTCACTACCAGTTTTAGCAGGGGCCATTACTATGTTATTAACAGACCGTAATCTGAACACCTCTTTTTTCGACCCTAGGGGGGGAGGAGATCCTATCTTGTACCAACACTTATTTTGATTTTTTGGTCACCCTTGAAGTTTAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Isopoda was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid