Armadillidium vulgare

April 2, 2025

Sample information
Picture
Photos by: Andrew R
Location
Collection date 03/11/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Caught in the wild during TJHSST stream field trip near Indian Run on March 11 2025
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Chopped roly poly in half and extracted DNA from front half of organism (including entire head and eyes and a fraction of its abdomen)

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia testing was done by a professional and not me
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
TGCTTGAGCAGGGGCTGTTGGAACAGCCCTTAGGATAATTATTCGAACTGAGTTAGGAAACCCTGGGAGGTTAATTGGAGACGATCAGATTTATAATGTGATTGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGCTTTGGTAATTGATTATACCTTTAATATTAGGGGCCCCTGATATAGCTTTTCCGCGTATAAATAATATAAGATTTTGGCTTCTTCCCCCATCACTAATTTTGTTATTAAGAAGAGGTTTAGTTGAAAGAGGTGTTGGGACTGGCTGGACTGTCTACCCACCTTTGGCAAGTGGAATTGCCCATAGAGGGGCTTCAGTAGATTTAGGGATTTTTTCCCTTCATTTGGCTGGAGCTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGAGCAGAGGGGATAAGGATAGATCGTGTCCCTCTATTTGTTTGGTCAGTGTTAATTACAGCAATTCTCTTGTTACTTTCACTACCAGTTTTAGCAGGGGCCATTACTATGTTATTAACAGACCGTAATCTGAACACCTCTTTTTTCGACCCTAGGGGGGGAGGAGATCCTATCTTGTACCAACACTTATTTTGATTTTTTGGTCACCCTTGAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Isopoda was found to be negative for Wolbachia.
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