Sample information |
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Picture |
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Location | |
Collection date | 03/12/2025 |
Captive / Cultivated? | Wild-caught |
Group | Thomas Jefferson High School for Science and Technology |
Observations | Wet, muddy, rocky, near stream, late winter, Beetle with a rough black exterior, long orange legs (with browning at the joints) and long orange antennae. Has six legs and a (relatively) large body compared to its head. |
Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
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Extraction kit | DNeasy (Qiagen) blood and tissue kit |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniPCR |
Buffer | TBE |
DNA stain | SYBR Safe |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | Unknown |
Confidence level | Medium |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary |