Thief Ant (Hymenoptera)

April 2, 2025

Sample information
Picture
Photos by: Jaslene H (experimented on by Saketha V)
Location
Collection date 03/12/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

The thief ant was caught near a stream, with lots of rocks, grass, wet soil, twigs, and sticks. It was late winter, at around 55 F, and it was found on the ground. It is small, brown, and has wings.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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