Woodlouse

April 2, 2025

Sample information
Picture
Photos by: Bhaavya J
Location
Collection date 03/09/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
TTTCTTCNATAAGAATAATTATTCTATTAGAATTAGGATCCCCTGATTCCCTAATTCTTAATGATCAAACCTTCATTACTATCGTTACTAGCCATGCTTTTATTATAATCTTTTTCATAGTTATACCTTTTATAATTGGAGGATTTGGTAACTTCTTANTACCCTTAATACTTGGGTCTCCTGATATANNCTATCCACGCCTAAATAACATAATATTCTGATTACTACCCCCTTCAATTTCTCTATTAATCTTAAGAAATTTTATTAATGAAGGATCAGGAACAGGTTGAACTATNTATCCCCCCCTATCATCTAATACCTTTNATAGTGGTCCCTCTATTGATTTAACTATTTTCTCCCTTCATATTGCCGGAATATCATCAATTCTAGGGGCAATCAATTTTATTTCAATNATTTTTAACATACATAATTCTAATATTACCTTAGATAAAATTCCCCTATTAGTATGATCTATTCTTATCACTGCTATTCTCCTCCTCCTCTCCCTTCCTATTCTTGCAGGAGCTATTACAATACTACTTACAGATCGAAATCTCAATACTTCNTTTTTTTTGATCCCTCAGGAGGAGGAGACCCTATTCTATATCAACACTTATTTTGATTTTNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
CTGGGAGTCTCATTGGGGATGATCAAATCTATAATGTAATTGTCACGGCTCATGCTTTTGTAATGATTTTCTTCATAGTAATACCTATTATAATTGGAGGTTTTGGAAATTGATTAATTCCTTTGATATTAGGGGCTCCTGATATAGCTTTCCCTCGTATAAACAATATAAGATTTTGGCTGCTTCCCCCTTCTTTAACTTTACTCCTTAGCAGAGGATTAATTGAAAGAGGAGTAGGAACGGGATGAACTGTTTACCCTCCTTTGGCATCAAGAATTGCCCATAGTGGAGCTTCTGTAGATTTAGGTATCTTTTCTCTTCATCTTGCTGGGGCTTCATCAATTTTAGGGGCAGTAAACTTTATTACGACTATAATTAATATACGGGCGTCCGGAATTAGTATGGATCGTGTGCCTTTATTTGTTTGGTCTGTTTTAGTAACCGCGGTTCTTCTTCTCTTATCTTTACCTGTTCTTGCTGGAGCTATTACTATACTATTGACTGACCGTAATTTAAATACATCTTTTTTTGATCCGAGGGGAGGGGGGGATCCTATTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Isopoda was found to be postive for Wolbachia.
Report Inappropriate Post