Aquarius remigis (Common Water Strider)

April 2, 2025

Sample information
Picture
Photos by: Joy P.
Location
Collection date 03/11/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

This bug was found in a clear, shallow stream near a bend with slower moving water.
Putative identification Arthropoda Insecta Hemiptera Gerridae Aquarius Aquarius remigis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Lab3_PCR_March2022-1

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

 

There was a clear band for both the CO1 and 16S tests which shows that this sample tested positive for Wolbachia. The controls also returned the expected results so contamination is unlikely.
Wolbachia 16S sequence Download AB1
GGGTGCGGATTTACCAGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCCATCGGGTAATGCTGAGCACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGCCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAAGCTAC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download AB1
TTTTTAGGTATATGATCAGGAATAATTGGAATATCTATAAGATGAATTATTCGAATTGAATTAGGTCAACCCGGATCTTTTATTGGAAATGATCAAATTTATAATGTAATTGTAACAGCCCATGCATTTATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTCGGAAATTGACTTGTACCTTTAATAATTGGAGCACCAGATATGGCATTTCCGCGAATAAATAATATAAGATTTTGACTTTTACCACCATCTTTAACTTTATTACTTGTTAGTAGTTTAGTTGACGCTGGAGCAGGTACTGGATGAACTGTTTATCCACCATTATCTAGAAATATTGCTCATAACGGAGCATCAGTAGATTTAACAATTTTCTCACTTCATTTAGCCGGTGTCTCATCAATTCTAGGAGCCGTAAATTTTATTTCAACTATCATTAATATACGAACAATAGGAATAACTAGAGAAAAAATTCCTCTTTTTGTTTGATCTGTAGGTATTACGGCATTACTTTTATTATTATCTTTGCCTGTTCTTGCAGGAGCAATCACAATATTATTAACAGACCGAAATTTAAATACATCTTTCTTTGATCCAGCTGGAGGAGGGGATCCAGTTCTTTACCAACATTTATTTTGATTTTTTGGTCACCCTTGAAGTTTAAA CAGCCCATGCATTTATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTCGGAAATTGACTTGTACCTTTA ATAATTGGAGCACCAGATATGGCATTTCCGCGAATAAATAATATAAGATTTTGACTTTTACCACCATCTTTAACTTTATT ACTTGTTAGTAGTTTAGTTGACGCTGGAGCAGGTACTGGATGAACTGTTTATCCACCATTATCTAGAAATATTGCTCATA ACGGAGCATCAGTAGATTTAACAATTTTCTCACTTCATTTAGCCGGTGTCTCATCAATTCTAGGAGCCGTAAATTTTATT TCAACTATCATTAATATACGAACAATAGGAATAACTAGAGAAAAAATTCCTCTTTTTGTTTGATCTGTAGGTATTACGGC ATTACTTTTATTATTATCTTTGCCTGTTCTTGCAGGAGCAATCACAATATTATTAACAGACCGAAATTTAAATACATCTT TCTTTGATCCAGCTGGAGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Aquarius remigis was found to be postive for Wolbachia.
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