Water Strider

April 2, 2025

Sample information
Picture
Photos by: Hrishi D.
Location
Collection date 03/12/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Collection location was a stream surrounded by several trees and rocks. Temperature was 44 F. Long, tapered, black body, long antennae + legs, small hairs covering body.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Gerridae Gerris Gerris marginatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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