Boye Zhu – Wolbachia DNA Barcoding Project

Sample information

Picture
Photos by: Lucas Z
Location
Collection date 03/12/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Long, thin body, small compound eyes at the end of the head, six legs, two distinctly larger and two smaller and folded up near the head, dark brown/black color, three-segmented body

Putative identification Arthropoda Hexapoda Insecta Hemiptera Gerridae Aquarius Aquarius remigis

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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