hymenoptera (carpenter ant)

April 2, 2025

Sample information
Picture
Photos by: Julia S.
Location
Collection date 03/09/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus castaneus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Both gel bands for CO1 and Wolbachia were bright red.
Wolbachia 16S sequence Download FASTA    Download AB1
TGGCTGTCGTCNGCTCGTGTCGTGAGNTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCTATCAGG TAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTAT GGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACA TCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGA ATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
ATTGGTTCTTCTATAAGAATAATTATTCGATTAGAATTAGGATCCCCTGATTCACTAATTCTTAATGATCAAACCTTCAA TACTATCGTTACAAGCCATGCTTTTATTATAATCTTTTTCATAGTTATACCTTTTATAATTGGAGGATTTGGTAACTTCT TAGTACCCTTAATACTTGGGTCTCCTGATATAGCCTATCCACGCATAAATAACATAAGATTCTGATTACTACCCCCTTCA ATTTCTCTATTAATCTTAAGAAATTTTATTAATGAAGGATCAGGAACAGGTTGAACTATTTATCCCCCCCTATCATCTAA TACCTTTCATAGTGGTCCCTCTATTGATTTAACTATTTTCTCCCTTCATATTGCCGGAATATCATCAATTCTAGGGGCAA TCAATTTTATTTCAACAATTTTAAACATACATAATTCTAATATTACCTTAGATAAAATTCCCCTATTAGTATGATCTATT CTTATCACTGCTATTCTCCTCCTCCTCTCCCTTCCTATTCTTGCAGGAGCTATTACAATACTACTTACAGATCGAAATCT CAATACTTCATTTTTTGANCCCTCANGANGNNGANACCCTATTCTATATCAACNCTTNNTTTGNNTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus castaneus was found to be postive for Wolbachia.
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