Monomorium minimum (A3 P7)

April 2, 2025

Sample information
Picture
Photos by: Jacqueline A.
Location
Collection date 03/09/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Monomorium

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Sample A3 in the gel images

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

This sample did not show a distinct band for either CO1 (arthropod barcoding) or Wolbachia. Since it was definitely an arthropod, this indicates that DNA was not extracted correctly and we cannot determine if this sample is positive or negative for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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