Odorous House Ant

April 2, 2025

Sample information
Picture
Photos by: Alexia R.
Location
Collection date 03/10/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

It was discovered at a very dry location, in the shade, more ants found by pulling up bark. Made the ant crawl onto leaf, alive during collection date.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
>7D4 ATAAGAATAATTATTCGTATTGAATTAGGGACCTGCGGAGCTTTAATTAATAATGATCAAATCTATAATTCAATTGTAAC AGGACATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGCTTTGGAAATTTTTTAGTCCCCTTAA TACTCGGAGCCCCAGATATAGCTTACCCCCGAATAAATAATATAAGATTTTGATTACTACCGCCCTCAATCCTTTTACTA ACTATTAGAAATTTTATCAGATCTGGGGTAGGGACCGGATGAACAGTTTACCCGCCCCTAGCATCTAATATCTATCATAA TGGACCTTCTGTAGATTTAGCTATTTTCTCCCTTCATATTGCTGGTATATCCTCAATTTTAGGGGCAATTAATTTTATTT CTACTATTATTAATATACACCATAAAAATTTTTCCACAGATAAAATCCCTCTTTTAGTATGATCAATCTTAATTACTGCA ATTTTATTACTCCTATCTCTCCCAGTTCTAGCTGGGGCTATTACTATACTTTTAACAGATCGAAACTTAAATACATCCTT TTTTGACCCTTCCGGAGGTGGGGACCCAATTTTATATCAACATTTATTTTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus was found to be negative for Wolbachia.
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