Common Water Strider 7B5

April 2, 2025

Sample information
Picture
Photos by: Tejeswini A.
Location
Collection date 03/12/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

It has long legs, its medium sized, and it has a dark color
Putative identification Arthropoda Hexapoda Insecta Hemiptera Gerridae Aquarius Aquarius nyctalis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
>7B5_WOLBACHIA ACAGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCCATCGGGTAATGCTGAGCACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGCCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
>7B5 GTAATATGATCAGGAATAATTGGAATATCTATAAGATGAATTATTCGAATTGAATTAGGTCAACCCGGATCTTTTATTGGAAATGATCAAATTTATAATGTAATTGTAACAGCCCATGCATTTATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTCGGAAATTGACTTGTACCTTTAATAATTGGAGCACCAGATATGGCATTTCCGCGAATAAATAATATAAGATTTTGACTTTTACCACCATCTTTAACTTTATTACTTGTTAGTAGTTTAGTTGACGCTGGAGCAGGTACTGGATGAACTGTTTATCCACCATTATCTAGAAATATTGCTCATAACGGAGCATCAGTAGATTTAACAATTTTCTCACTTCATTTAGCCGGTGTCTCATCAATTCTAGGAGCCGTAAATTTTATTTCAACTATCATTAATATACGAACAATAGGAATAACTAGAGAAAAAATTCCTCTTTTTGTTTGATCTGTAGGTATTACGGCATTACTTTTATTATTATCTTTGCCTGTTCTTGCAGGAGCAATCACAATATTATTAACAGACCGAAATTTAAATACATCTTTCTTTGATCCAGCTGGAGGAGGGGATCCAGTTCTTTACCAACATTTATTTTGATTTTTTGGTCACCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Aquarius nyctalis was found to be postive for Wolbachia.
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