Wolbachia Explored in Hawai’i (Mosquito Negative)

April 3, 2025

Sample information
Picture
Location
Collection date 01/31/2025
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations
Putative identification

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Collected manually by hand in Aina loa, Hawai’i.

Top Row C01(Wells 1-8) includes in order; DNA Ladder, Ant 1,Ant 2, Ant 3, Ant 4, Ant 5,Ant 6, Mosquito 1 (M1).

Second row (Wells 9-18) includes in order; DNA Ladder, Ant 1,Ant 2, Ant 3, Ant 4, Ant 5,Ant 6, Mosquito 1 (M1).

Third Row C01 Controls(Wells 16-21) includes in order; DNA ladder, DNA+, Diptera+, Diptera-, H20

Fourth Row 16S/Wolbachia  Controls(Wells 25-29)includes in order; DNA ladder, DNA+, Diptera+, Diptera-, H20

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls and samples were processed accordingly. Wolbachia was present for ant species but not in mosquito
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary
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