Stripe-eyed flower fly, Eristalinus taeniopa

April 3, 2025

Sample information
Picture
Photos by: Abigail N.
Location
Collection date 02/02/2024
Captive / Cultivated? Captive / Cultivated
Group Hawaii Community College
Observations
Putative identification Arthropoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

200 voltage, 2% agarose, below is gel electrophoresis map

Ladder CLO 1 CLO 2 CLO 3 CLO 4 CLO 5 + –
Ladder (+) DNA Control (-) Water
Ladder W 1 W 2 W 3 W 4 W 5 + –
Ladder (+) DNA Control (-) Water

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The positive and negative controls worked showing the data is accurate.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
Report Inappropriate Post