Stripe-eyed flower fly, Eristalinus taeniopa

Sample information

Picture

Photos by: Abigail N.

Location

Collection date

02/02/2024

Captive / Cultivated?

Captive / Cultivated

Group

Hawaii Community College

Observations

Putative identification

Arthropoda

Methods

Extraction kit

Edwards Buffer

DNA extraction location

Whole arthropod

Single or Duplex PCR

Single Reaction

Gel electrophoresis system

Edvotek Gel Electrophoresis

Buffer

TBE

DNA stain

SYBR Safe

Gel images

Protocol notes

200 voltage, 2% agarose, below is gel electrophoresis map

Ladder	CLO 1	CLO 2	CLO 3	CLO 4	CLO 5	+	–
Ladder	(+) DNA Control	(-) Water
Ladder	W 1	W 2	W 3	W 4	W 5	+	–
Ladder	(+) DNA Control	(-) Water

Results

Wolbachia presence

Confidence level

Medium

Explanation of confidence level

The positive and negative controls worked showing the data is accurate.

Wolbachia 16S sequence

Arthropod COI sequence

Download FASTA Download AB1

GTATTCTAATCCGAGCTGAACTGGGTCATCCTGGTGCTTTAATTGGAGACGACCAAATTTATAATGTAATCGTCACTGCACATGCTTTTGTTATAATTTTTTTTATAGTGATACCTATTATAATCGGTGGGTTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGACTTCTTCCCCCTGCTTTATCACTTTTATTAGTAAGAAGTATAGTTGAAAACGGAGCTGGGACAGGGTGAACTGTTTACCCACCTCTATCATCAGGAATCGCCCATGGAGGAGCTTCTGTAGATTTAGCTATTTTTTCTTTACATTTAGCCGGAATCTCTTCAATTTTAGGGGCAGTTAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTACTCTTGATCGTATACCTTTATTTGTTTGGTCAGTCGTAATTACTGCACTTTTATTATTATTATCTCTTCNAGTTTTAGCCGGGGCTATTACTATGTTACTAACTGATCGAAATTTAAACACATCATTTTTTGACCCTGCAGGAGGAGG

BLAST at The Wolbachia Project BLAST at NCBI

Summary

The Arthropoda was found to be negative for Wolbachia.

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