Armadillidium nasatum (Nosy Pill Woodlouse)

April 7, 2025

Sample information
Picture
Photos by: Nathan L.
Location
Collection date 03/11/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Found under a log near Indian Run Stream off of Randolph rd.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium nasatum

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Both of our positive and negative controls worked, and all of the arthropods run on the same gel were positive for arthropod CO1 DNA. The DNA purity was acceptable but not ideal, with a 260/280 ratio of 1.59.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium nasatum was found to be negative for Wolbachia.
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