April 16, 2025

Sample information
Picture
Photos by: Lana E
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 student
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My controls worked as expected. The positive control showed clear bands on the gel, indicating that the PCR amplification for Wolbachia was successful and the reagents were functioning properly. The negative control did not produce any bands. Both controls worked correctly, I can trust that my sample’s negative result for Wolbachia was accurate without any contamination.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
AGCATGGATGTTGATAGGATAGGGTCTCCTCCTCCCCTAGGGTCAAAAAAAGAGGTATTTAAATTTCGATCAGTTAATAATATAGTAATAGCTCCTGCTAATACAGGTAGGGATAAAAGTAAAAGTACAGCCGTTACTAATACTGATCAAACAAATAAAGGAACACGGTCTATTCTAATTCCAGCTGCTCGTATATTAATTATAGTAGTAATAAAATTCACAGCCCCTAAAATAGAAGAAGCTCCAGCAAGGTGGAGAGAAAAAATACCTAAATCTACAGATGCTCCTCTATGAGCAATTCTAGACGCCAGCGGAGGATATACTGTCCATCCTGTTCCTACTCCTCTTTCAACCAACCCACTTCTAAGCAATAAAGTTAAAGAAGGGGGTAAAAGTCAAAATCTTATATTATTTATTCGTGGAAAAGCCATATCTGGGGCTCCTAGTATTAATGGAATTAACCAATTACCAAATCCACCAATTATAATAGGTATTACTATAAAAAAAATTATTACAAAAGCATGAGCAGTTACAATTACATTGTAAATCTGGTCATCTCCAATTAAACTTCCAGGTTGTCCTAATTCAGTGCGAATAATTATTCTAAGGGCAGTTCCAACAGCCCCTGCTCAAACCCCAAATACAAAATATAAAGTTCCAATATCTTTATGTTTTGTTGGACCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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