April 15, 2025

Sample information
Picture
Location
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • We used a dip net
  • Gloved hands
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I can trust my results due to the controls being accurate .
Wolbachia 16S sequence
Arthropod COI sequence
GCTTGATCAGGAATAGTAGGAACTTCACTTAGTATACTTATTCGAGCGGA ACTtcctttacgcggATCTCTTATTGGAGATGATCAAATCTATAATGTAA TTGTAACAGCACACGCCTTCATTATAATTTTCTTTATAGTAATACCCATT CTAATTGGTGGATTTGGAAATTGACTAGTTCCTCTTATATTAGGAGCACC AGATATAGCCTTTCCACGGATAAATAATATAAGATTCTGATTATTACCTC CTTCTTTGACCCTGCTATTAGCTAGAAGAATAGTAGAAAGTGGAGCTGGT ACTGGATGAACAGTCTACCCTCCACTTGCAAGAAGTATTGCACATGCTGG AGCATCTGTAGATCTTGCTATTTTTTCTCTACATCTCGCAGGAGTATCTT CAATTCTAGGAGCAGTAAACTTTATCTCAACAATTATTAATATAAAACCA ATTAGTATAAAACCTGAACGTATTCCTTTATTTGTATGAGCTGTTGGAAT TACTGCTTTACTTCTTCTTTTATCCTTACCAGTCCTTGCAGGGGCAATTA CTATACTTCTTACTGATCGAAATTTAAATACCTCCTTCTTTGACCCTGCT GGAGGAGGAGATCCAATTCTTTACCAACATTTATTTTGATTTTTTGGTCA CCCTGAAGTTTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be negative for Wolbachia.
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