Field Ant

April 12, 2025

Sample information
Picture
Photos by: Ayesha Waheed
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica Formica pallidefulva

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I had a strong arthropod gel band, but no Wolbachia gel band
Wolbachia 16S sequence
Arthropod COI sequence
ATAATATGTTGATATAGATTGGGTCCCCCCTCCTGAAGGGTCAAAAAATGAAGTATTTAAGTTTCGGTCAGTTAATAATATTGTAATAGCCCCAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTGCAGTGATTAAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTAAGAATTGTTGAAATAAAATTAATAGCCCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGTCCATTATGAAAAATATTTGAAGCTAAAGGAGGGTAGATAGTTCATCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTGATGGAAGGAGGTAAAAGTCAAAATCTTATATTATTTATACGAGGATAAGCTATATCTGGTGACCCTAATATTAAAGGAATTAGAAAATTTCCAAATCCACCAATTATAAATGGTATTACTATAAAAAAGATTATAATAAAGGCATGATTAGTTACTAAAGAATTATAAATTTGATCATTATTAATTAATGAATTTGAAGAACCTAATTCTAATCGAATAATTATACTTATAGAAGATCCAATTATTCCAGCTCAGATAGCAAATAAAAAATAAAGAATTCCAATATCTTTATGATTTTGTTGACCCAATAATATGTTGATATAGATTGGGTCCCCCCTCCTGAAGGGTCAAAAAATGAAGTATTTAAGTTTCGGTCAGTTAATAATATTGTAATAGCCCCAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTGCAGTGATTAAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTAAGAATTGTTGAAATAAAATTAATAGCCCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGTCCATTATGAAAAATATTTGAAGCTAAAGGAGGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formica pallidefulva was found to be negative for Wolbachia.
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