DEM-GTL/Blattodea

April 14, 2025

Sample information
Picture
Photos by: Gabrielle L.
Location No map location data available.
Collection date 01/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

• Insect collected on 1/27/25 at 2:00:00 PM
• Georgia Southern University (Armstrong Campus)
• Used swift force on trees nearby body of water
• Gloved hands used to break open trees near pond
• Insect found within branches using gloved hand method
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Even though the results are not what we predicted, I am still confident that they are interpreted correctly.
Wolbachia 16S sequence
Arthropod COI sequence
TGGATAGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCA ACCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGG CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be negative for Wolbachia.
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