DEM-SEC Hymenoptera

April 14, 2025

Sample information
Picture
Photos by: Sara C.
Location No map location data available.
Collection date 01/19/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

caught on a tree using a collection jar.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location In front of University Building
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

insects’ Order is Hymenoptera, and the likelihood of them being infected with Wolbachia is very low, so it fits the results on the gel electrophoresis that the insect is negative for Wolbachia.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
ATAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTC AAATTCATTAATTAATAATGATCAAGTTTATAATTCTATAGTTACTAGAC ATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGA TTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCCCCAGATATAGCCTA TCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTT TACTTTTATTAAGAAATTTCATTAATGATGGAGTGGGAACAGGATGAACT GTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGCCCTTCAGTTGA CTTAACAATTTTTTCTCTTCATATTGCGGGAATATCTTCTATTCTAGGAG CTATCAATTTTATTTCAACAATTTTAAATATACGACATAAAAATTTTTCT TCTGATAAAATTCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTT ACTTCTTCTTTCTTTACCAGTTCTTGCTGGAGCTATTACTATACTTTTAA CTGACCGAAATCTTAATACTTCCTTTTTTGATCCCTCAGGCGGAGGAGAC CCTGTTCTTTATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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