DEM-ACT

April 14, 2025

Sample information
Picture
Photos by: Ashley T
Location No map location data available.
Collection date 01/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • 01/27/2025
  • 2:15pm
  • Outside of Science Center
  • Parking lot
  • Gloves
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Based off of my gel electrophoresis results and having my DNA sent for sampling, I am confident my insect was no infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
GTAGGAACTTCACTTAGTATACTTATTCGAGCAGAACTTAATctaCCCGG ATCTCTTATTGGGGATGATCAAATTTATAATGTTATTGTAACAGCACACG CCTTCATTATAATTTTCTTTATAGTAATACCCATCCTAATTGGTGGGTTT GGGAATTGGCTAGTTCCTCTTATATTAGGAGCACCAGATATAGCTTTTCC ACGGATAAATAATATAAGATTCTGATTATTACCTCCTTCTTTGACCTTGC TGCTGGCTAGAAGAATAGTAGAAAGTGGAGCTGGTACTGGATGAACAGTT TATCCTCCACTTGCAAGAAGTATTGCACATGCTGGAGCATCTGTAGATCT TGCTATTTTTTCTCTACACCTTGCAGGAGTATCTTCAATTCTAGGGGCAG TAAACTTTATCTCAACAATTATTAATATAAAACCAATCAGTATAAAACCT GAACGTATTCCTTTATTTGTATGAGCTGTTGGAATTACTGCTTTACTTCT TCTTTTATCCTTACCAGTCCTTGCAGGAGCAATTACTATACTTCTTACTG ATCGAAATTTAAATACCTCCTTCTTTGACCCTGCTGGAGGGGGAGACCCT ATTCTTTACCAACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be negative for Wolbachia.
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