Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/27/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Dermaptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My results were clear to show that my insect was infected with Wolbachia
|
| Wolbachia 16S sequence |
AGacCCGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGA TTCCAACTttaTGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGC TTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACC ATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACAT CATCCCCACCTTCCTCCAGTTTATCACTAGCAGTTTCCTTAAAGTCCCCA GCATTACCTGATGGTAACTAAGGATGAGGGTTACGCTCGTTGCGGGACTT AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGT GAAATCCGGCCGAACCGACCCTATCCCTTCgaaTAGGTATG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
|
| Summary | The Dermaptera was found to be postive for Wolbachia. |
Phymata americana
WRITNA-12-COLEOPTERA
Harmonia axyridis
GRE-LCH Coleoptera
Apis Mellifera