Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | There was no Wolbachia band when the gel electrophoresis was completed. Though, this may be due to errors in extraction and DNA replication, which may have provided a false negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
|
| Summary | The Melanoplus was found to be negative for Wolbachia. |
Oncopeltus fasciatus
Formica Pallidefulva
Hylephila Phyleus
Thomisus onustus
Halictus Ligatus