Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 01/28/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Cold day, sun out, grassy field |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I was not expecting Wolbachia to be present and my results matched. I am also very confident that there were no egregious mistakes made during the experiment. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TAATTGGATCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTGT AATTCTCTAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACA CGCTTTTATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGAT TTGGAAATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTAT CCTCGAATAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCT TTTACTTATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTA TCTACCCACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGAT CTATCTATTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGC TATTAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTA TAGATAAAATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTT CTTTTACTTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAAC TGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATC CCATTCTATACCAACATTTATTCTGATTTTTTGGTC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Oncopeltus fasciatus
Formica Pallidefulva
Hylephila Phyleus
Thomisus onustus
Halictus Ligatus