Arrup Chamberlin (soil centipede)

September 13, 2026

Sample information
Picture
Location No map location data available.
Collection date 11/01/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

random insect given by professor
Putative identification Arthropoda Chilopoda Geophilomorpha Mecistocephalidae Arrup

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

top row

DNA Standard
AYPHF1 + AYPHF7
AYPHF2 + AYPHF8
AYPHF3 + AYPHF9
AYPHF4 + AYPHF10
AYPHF5 + AYPHF11
AYPHF6 + AYPHF12
empty

bottom row

DNA Standard
VALEE1 + VALEE7
VALEE2 + VALEE8 – pictured insect
VALEE3 + VALEE9
VALEE4 + VALEE10
VALEE5 + VALEE11
VALEE6 + VALEE12
empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No approx. 400 BP band to signify Wolbachia presence
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TNNNTTTCGGGGCCTGATCTGCTATAATAGGAACGGCCCTTAGACTCCTAATCCGCTTAGAATTAAGCCAACCAGGAAGC TTAATCGGAGATGACCAAACATACAACGTAGTAGTCACAGCTCACGCATTCGTAATAATCTTCTTCATAGTAATGCCTAT TATAATAGGAGGATTTGGCAATTGACTATTACCCCTAATGTTGGGGGCCCCTGACATAGCCTTTCCCCGACTTAATAATA TAAGCTTCTGGCTACTCCCCCCATCCCTAACTCTACTGTCTGCCTCCGCAGCTGTAGAGAGTGGGGCAGGAACAGGCTGA ACTGTATACCCCCCCCTGTCATCTGGAATTGCCCATTCCGGAGCCTCAGTAGATATAACAATTTTCTCCCTTCATCTTGC CGGAGTATCTTCAATTTTAGGGGCCGTAAATTTCATTACAACAGTAATTAATATACGAACAAGCGGAATAGTAATAGAGC GAGTCCCCCTATTCGTATGGGGGGTTGTAATTACAGCAATTCTTCTTCTTTTATCCCTCCCTGTTCTAGCTGGAGCAATT ACAATACTTCTCACCGACCGCAACTTTAATACCAGTTTCTTTGACCCTGCAGGAGGAGGAGACCCTATTTTATACCAGCA CCTATTCTGATTTTNNNNNNNCCNNANNNNNNNNAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arrup was found to be negative for Wolbachia.
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