WRIMRS-19 Blattodea

April 16, 2025

Sample information
Picture
Photos by: Morrow S.
Location No map location data available.
Collection date 01/15/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found in damp topsoil among leaves.
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The sample’s purity and comparison with classmates make me confident in my results.
Wolbachia 16S sequence
CATACCTATTCGAAGGGATAGGGTCGGTTCGGCCGGATTTCACACAGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGTAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGATTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATAC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
ATAGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTaaccctatc tGgTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTC ACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGAGGA TTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATT TCCTCGTATAAATAATATAAgtTTTTGACTTTTACCTCCATCTCTATCTC TTCTATTAGCCagtaaccTTGTCGAAAGAGGAGCTGGGACTGGTTGAACC gTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGA TTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAGGTG CCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATAAGC CCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATTATT ATTATTATTATCCTTACCAGTTCTTGCaggTGCAATTACAATATTATTAA CAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGgagAT CCTATTTTATATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be negative for Wolbachia.
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