Vespula maculifrons

April 16, 2025

Sample information
Picture
Photos by: Natalia Z.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I chose medium because because I did not have a thick gel band but I still got very good BLAST results in the end. My species also did not have wolbachia.
Wolbachia 16S sequence Download FASTA    Download AB1
>WSpecF.ab1 (341 bp) GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCC AGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGG TGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTC GAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGC CCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>11-C_AP3_Co1_R.ab1 (610 bp) GTTTAAGTTTCGATCTGTTAAAAGTATAGTAATTGCTCCTGCAAGAACTGGTAGTGATAATAGAAGAAGAATTGCTGTAA TTAAAATTGATCATGTGAATAAAGGAAGAAAATTTAATGAATGTGTTTTTGTGTGTATATTTAAAATAGTAACGATAAAA TTAATTGATCCTATAATTGATGAAATTCCAGCAATATGAATTGAAAAAATTCCTAAATCTACAGATGGAGAATCATGACC AACAATAGAAGACAAAGGAGGGTATAAAGTTCATCCTGTTCCTACCCCTGTTCCAATAAAATTTCTTAAAATTAGTAAAA ATAATGAAGGAGGGAGTAATCAAAATCTTATATTATTTATTCGAGGAAATGCTATATCAGGTACACCTAGTATTAAAGGG ATTAATCAGTTTCCAAATCCTCCTACTAAAAAAGGTATAACTATAAAGAAAATTATAATAAAGGCATGAGCTGTAATAAT TGTATTATAAATTTGATCATTATTAATTAAGGCCCCAGGAGATCTTAATTCTAAGCGAATAATTATTCTTATTGAAGCTC CTAAAGTCCCTGCCCATAAAGCAAAAATAAAATATAATGTTCCAATATCT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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