BRO-CMT

April 17, 2025

Sample information
Picture
Photos by: Cayla T.
Location
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Collecting insects that may contain Wolbachia
  • Collected ants, lady bug, nats
  • Gathered leaves, dry grass, dirt, rocks came with our samples
  • Samples were taken at about 10:20 am – 10:40 am
  • Samples taken around grassy sides of roads
  • Also in tall dry grass or under logs
  • Used a sweep net 
  • Borrowed a hit stick
  • Samples were found in damp shady places
Putative identification Arthropoda Collembola

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 0.25 TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My DNA didn’t match with the Wolbachia 16S gene after it was ran through gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
TTtGATcGGCTATGATTGGTACTGCTTTTAGTGTATTAATTCGTTTAGAA CTTGGtctacCGGGTAGTTTAATTGGTGATGATCAGATATACAATGTTTT AGTAACATCCCATGCATTTATTATGATTTTCTTTATGGTTATACCCATCA TAATTGGAGGTTTCGGTAACTGACTAGTACCTCTTATGATTGGAGCACCT GATATGGCTTTTCCGCGGATAAATAATATAAGGTTTTGACTATTACCTCC CGCCCTTACTCTTTTATTAATAGGGGGTATAGTGGAAAGAGGTACAGGAA CAGGATGAACTGTGTACCCCCCACTAGCTTCTTCTATCTCACACTCTGGG GCATCAGTAGATTTAACTATTTTTAGTCTTCATTTAGCTGGTGTTTCATC TATTTTAGGTGCTATTAATTTTATTACAACAATTATTAATATACGAGCCC CAGGAATAAACTGAGATTTAACACCTCTTTTTGTGTGGTCTGTCTATATT ACTGCAATTTTACTTCTCCTTTCACTACCAGTTTTAGCTGGTGCCATTAC AATACTTTTAACTGATCGAAATTTAAATACTACATTTTTTGATCCAGCTG GGGGGGGTGATCCTATTTTATATCAACATCTGTTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Collembola was found to be negative for Wolbachia.
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