BRO-BET Order Collembola

April 17, 2025

Sample information
Picture
Photos by: Bryn T.
Location
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
 

Collection Date

  

1/30/2025
 

Collection Time

  

10:30 am
 

Collection Method

  

Aerial/Sweep Net
 

Location of Collection

 Georgia Southern University Armstrong Campus – near police station and greenhouse
 

Description of Habitat

 Dry grass, wet mud under logs, small brush area with leaves
Putative identification Arthropoda Collembola

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 0.25 TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Gel electrophoresis data did not support the prediction of Wolbachia infection in the insect sample. Locally collected Collembola data had a 25% presence of Wolbachia infection. The data results displayed the insect sample as negative for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
GAGTTTGATcGGCTATGATTGGTACTGCTTTTAGTGTATTAATTCGTTTA GAACttcgtcaaccGGGTAGTTTAATTGGTGATGATCAGATATACAATGT TTTAGTAACATCCCATGCATTTATTATGATTTTCTTTATGGTTATACCCA TCATAATTGGAGGTTTCGGTAACTGACTAGTACCTCTTATGATTGGAGCA CCTGATATGGCTTTTCCGCGGATAAATAATATAAGGTTTTGACTATTACC TCCCGCCCTTACTCTTTTATTAATAGGGGGTATAGTGGAAAGAGGTACAG GAACAGGATGAACTGTGTACCCCCCACTAGCTTCTTCTATCTCACACTCT GGGGCATCAGTAGATTTAACTATTTTTAGTCTTCATTTAGCTGGTGTTTC ATCTATTTTAGGTGCTATTAATTTTATTACAACAATTATTAATATACGAG CCCCAGGAATAAACTGAGATTTAACACCTCTTTTTGTGTGGTCTGTCTAT ATTACTGCAATTTTACTTCTCCTTTCACTACCAGTTTTAGCTGGTGCCAT TACAATACTTTTAACTGATCGAAATTTAAATACTACATTTTTTGATCCAG CTGGGGGGGGTGATCCTATTTTATATCAACATCTGTTTTGATTTTTTGGT CACCCTGAAG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Collembola was found to be negative for Wolbachia.
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