BRO-TRS Blattodea

April 17, 2025

Sample information
Picture
Location No map location data available.
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Specimen found in refuse area near the established collection site
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

After PCR a near pure sample was obtained and after electrophoresis process the bp of Wolbachia was detected.
Wolbachia 16S sequence
Arthropod COI sequence
TGGATAgtagGGACATCTTTAAGAATATTAATCCGAGCAGAACTAaatca tcCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGG CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTCACCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be postive for Wolbachia.
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