BRO-ML BLATTODEA

April 17, 2025

Sample information
Picture
Photos by: Mylan L.
Location
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

01/30/2025
10:50 am
Outside the science building, near the university garden, Georgia Southern Armstrong campus
Bushes, Trees, and Straw
Sweeping net
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

After conducting my experiment, my sample was positive for C01 insect gene, but not the 16s Wolbachia gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Blattodea was found to be negative for Wolbachia.
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