Hylephila phyleus

April 20, 2025

Sample information
Picture
Photos by: Sarah S.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students
Putative identification Arthropoda Insecta Lepidoptera Hesperiidae Hylephila Hylephila phyleus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Medium because my Wolbachia gel band has a %ID match greater than 99.00%. It came out to be 99.84%
Wolbachia 16S sequence
>w19R.ab1 (359 bp) CCGTGGCGTGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGG CTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATA AGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCT GATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGC AACACCTGTGTGAAATCCGGCCGAACCGACCCTATCCCT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
>46-D_AP3_Co_R.ab1 (608 bp)AAAAAGATGTATTTAAATTTCGATCAGTTAATAATATAGTAATAGCTCCAGCTAAAACAGGTAAAGATAATAATAATAATAATGCAGTAATACCTACAGATCATACAAATAATGGTATTTGATCAAATGATAAATTTTTAATTCGTATGTTAATAATTGTTGTAATAAAATTAATAGCTCCTAAAATAGATGAAATTCCAGCTAAATGAAGGGAAAAAATTGCTAAATCAACAGAAGATCCTTGATGAGCAATATTAGAAGATAGAGGGGGGTAAACTGTTCATCCTGTTCCTGCACCATTTTCTACAATTCTTCTTGAAATTAATAAAGTTAATGAAGGGGGTAGTATTCAAAATCTTATATTATTTATTCGAGGAAATGCTATATCAGGAGCTCCTAATATTAAAGGTACTAATCAATTACCAAATCCTCCAATTATAATTGGTATTACTATAAAGAAAATTATAATGAAAGCATGAGCTGTAACAATAGTATTATAAATTTGATCATCTCCAATTAAAGATCCAGGATTACCTAATTCTGTTCGAATTAATAAACTTAAGGAAGTTCCTAATATTCCTGCTCAAATACCAAAAATAAAATATAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hylephila phyleus was found to be negative for Wolbachia.
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