Formica pallidefulva

April 20, 2025

Sample information
Picture
Photos by: Dunia E
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica Formica pallidefulva

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am highly confident that my arthropod has Wolbachia because the BLAST result showed a 100% ID match for Wolbachia.
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download FASTA   
Summary The Formica pallidefulva was found to be postive for Wolbachia.
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