Sample information
Picture	Photos by: Zalfa S.
Location
Collection date	09/03/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by Fall 2024 students.
Putative identification	Arthropoda Hexapoda Insecta Diptera Syrphidae Eupeodes Eupeodes americanus
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	body without wings
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	Did not have a Wolbachia band in gel electrophoresis.
Wolbachia 16S sequence	Download FASTA
Arthropod COI sequence	Download FASTA
Summary	The Eupeodes americanus was found to be negative for Wolbachia.