Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes fuscatus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The bands in the gel results were very dark and strong, suggesting that the results that came forward from the sequencing of the gel are reliable/accurate. In the confidence of these results, it can be observed that there was no Wolbachia present in the DNA of the arthropod, as the bands of the arthropod DNA did not match with those of the positive Wolbachia control. |
| Wolbachia 16S sequence |
GGGTCGTCAGGCAGTGTGTACAGACCCGAGAACGTATTCACCGTGGCGTGCTGATCCACGATTACTAGCGATTCCAACTT CATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGGACTTTGCAGCCC ATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCA GTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGAC TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAATCCGGCCGAACCGACCCTATCCCT TCGAAAAAGGTATGAG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
AGAAAGATGCTTGTCATTCTTAGTGATCTCCAACTCCTAATGGGTCAAAAAAAGATGTATTAATATTTCGATCAGTTAAT AGTATGGTAATTGCTCCAGCTAGAACAGGTAAAGATAATAAAAGTAAAATTGCAGTAATTAAAACTGATCATGTAAATAT AGGTAAAAAATTTAATGAATGAGTTTTAATATGTATATTTAAGATTGTTACAATGAAATTAATAGCTCCTATGATTGATG AAATTCCTGCAATGTGAAGAGAGAAAATAGCAAAATCAACTGATGATGAATTGTGACCTATATTAGATGATAAAGGTGGA TATAAAGTTCATCCAGTTCCTACTCCTATATCATTAATATTTCTAATAATTAATAATAATAATGATGGTGGTAATAATCA AAATCTTATATTATTTATTCGTGGGAATGCTATATCTGGGGCACCTAGTATTAAGGGAATTAATCAATTTCCAAATCCTC CAATTATAAATGGTATAACTATGAAAAAAATTATAATTAATGCGTGAGCTGTAATAATTGAATTATAGATTTGATCATTA TTAATAATTGATAAAGGTGTACCTAATTCTAGACGAATAATTAATCTTAATGATCTTCCAATTATACCAGCTCATAGACC AAAGATAAAATATAAAGTTCCAATATCTTTATGTTGGTGTTGAAAACA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Polistes fuscatus was found to be negative for Wolbachia. |
Ladybug
Armadillidium vulgare #1 GRP30
Drosophila Melanogaster
Armadillidium vulgare GRP7 (1)