Vespula germanica

April 22, 2025

Sample information
Picture
Photos by: Dr. Cheryl Heinz
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Vespidae Vespula Vespula germanica

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I had no band for Wolbachia in the gel electrophoresis.
Wolbachia 16S sequence
Didn't have one
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
>63-D_AB1_Co1_R (1).ab1 (671 bp) GGAAATGATGATATAAATTGGGTCCCCCCACCTGCAGGATCGAAAAAAGATGTGTTTAAGTTCCGATCTGTTAAAAGTAT AGTAATTGCTCCTGCAAGAACTGGTAGTGATAATAGAAGAAGAATTGCTGTAATTAAAATTGATCATGTGAATAAAGGAA GAAAATTTAATGAATGTGTTTTTGTGTGTATATTTAAAATAGTAACGATAAAATTAATTGATCCTATAATTGATGAAATT CCAGCAATATGAATTGAAAAAATTCCTAAATCTACAGATGGAGAATCATGACCAACAATAGAAGATAAAGGAGGGTATAA AGTTCATCCTGTTCCTACCCCTGTTCCAATAAAATTTCTTAAAATTAGTAAAAATAATGAAGGAGGGAGTAATCAAAATC TTATATTATTTATTCGAGGAAATGCTATATCAGGTACACCTAGTATTAAAGGGATTAATCAGTTTCCAAATCCTCCTACT AAAAAAGGTATAACTATAAAGAAAATTATAATAAAGGCATGAGCTGTAATAATTGTATTATAAATTTGATCATTATTAAT TAAGGCCCCAGGAGATCTTAATTCTAAGCGAATAATTATTCTTATTGAAGCTCCTAAAGTCCCTGCCCATAAAGCAAAAA TAAAATATAATGTTCCAATATCTTTATGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Vespula germanica was found to be negative for Wolbachia.
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