Sample information
Picture	Photos by: Suhaib S.
Location
Collection date	09/03/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	collected by Fall 2024 students
Putative identification	Arthropoda Arachnida Araneae Theridiidae Parasteatoda Parasteatoda tepidariorum
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	I wasn’t able to get a usable band for Wolbachia unfortunately
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA
Summary	The Parasteatoda tepidariorum was found to be negative for Wolbachia.