Boettcheria cimbicis

April 22, 2025

Sample information
Picture
Photos by: Rayaan I
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda Insecta Diptera Sarcophagidae Boettcheria Boettcheria cimbicis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Have high confidence, no band was formed for Wolbachia, and the controls were correct.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
Summary The Boettcheria cimbicis was found to be negative for Wolbachia.
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