Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | collected by Fall 2024 students |
| Putative identification | Arthropoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes fuscatus |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No Wolbachia bands were shown for the DNA. However, the arthropod DNA and the controls all had the expected results which is why these results can be trusted. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
>56-D_AOR4_Co1_R.ab1 (588 bp) TATTAATATTTCGATCAGTTAATAGTATGGTAATTGCTCCAGCTAGAACAGGTAAAGATAATAAAAGTAAAATTGCAGTA ATTAAAACTGATCATGTAAATATAGGTAAAAAATTTAATGAATGAGTTTTAATGTGTATATTTAAGATTGTTACAATGAA ATTAATAGCTCCTATGATTGATGAAATTCCTGCAATGTGAAGAGAGAAAATAGCAAAATCAACTGATGATGAATTGTGAC CTATATTAGATGATAAAGGTGGATATAAAGTTCATCCAGTTCCTACTCCTATATCATTAATATTTCTAATAATTAATAAT AATAATGATGGTGGTAATAATCAAAATCTTATATTATTTATTCGTGGGAATGCTATATCTGGGGCACCTAGTATTAAGGG AATTAATCAATTTCCAAATCCTCCAATTATAAATGGTATAACTATGAAAAAAATTATAATTAATGCGTGAGCTGTAATAA TTGAATTATAGATTTGATCATTATTAATAATTGATAAAGGTGTACCTAATTCTAGACGAATAATTAATCTTAATGATCTT CCAATTATACCAGCTCATAGACCAAAGA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Polistes fuscatus was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid