Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | I found it in a little forest. It was at about 3pm. It was under a conifer bark. It was 10 to 15 degrees. There were 4 of this bugs but I took one. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Cerambycidae Rhagium Rhagium inquisitor |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The experiment worked there were the CO-DNA band. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence |
|
| Summary | The Rhagium inquisitor was found to be negative for Wolbachia. |
Ladybug
Armadillidium vulgare #1 GRP30
Drosophila Melanogaster
Armadillidium vulgare GRP7 (1)