Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | I found it at a little forest. It was about 3 pm, with 10-15 degrees. Under a dry conifer in the shadow. There were alot of them there. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Porcellionidae Porcellio Porcellio scaber |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Everything went well, i got a result and had no problems with the procedure |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence |
|
| Summary | The Porcellio scaber was found to be negative for Wolbachia. |
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