Ant

May 1, 2025

Sample information
Picture
Photos by: Mahnoor K.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The reason I selected “Medium” for the confidence level is because my insect had a strong Wolbachia gel band, however, it had a BLAST match between 99.00% – 99.99% of 99.56%.
Wolbachia 16S sequence Download FASTA    Download AB1
TGGTTGTATCTCGGGGTTGACAGAACCGAAGAACGTATTCACCGTGGCGTGCTGACCACGATTCCTCGCGATTCAACTCCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAATCCGGCCGAACCGACCCTATCCCTTGACTGCGTATGCCAGAACCCGCTATCCCTGAGCCCCGCCTTATCGTAGGAAAATCATGTGCGCAAAAGGCCAGCAATAGGACAGGATACTATAAAAAGAATCGTTGTGGGAGTTGACCATTAGCTCCTCCCCCCTGACTAGGATCGATTACAATTAATCTTCCTCTATAAGTGGCGTATCCCTAAATGACTATAAAGATAGAAGGCGCTACCATCTGGACCCTCCCTCGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TATCGTTGGTTGATTCAGAATCGGGTCTCCCCTCCTGAAGGGATCAAAAAATGAAGTATTTAAGTTTCGATCAGTTAATAATATTCGTAATAGCTCCAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTCGCAGTGATTAAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTAAGGATTGTTGAAATAAAATTAATAGCTCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGCCCATTATGAAAAATATTTGAAGCTAAAGGAGGGTAGATAGTTCACCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTGATGGAAGGAGGTAAAAGTCAAAATCTTATATTATTTATACGAGGATAAGCTATATCTGGCGATCCTAATATTAAAGGAATTAGAAAATTTCCAAATCCACCAATTATAAATGGTATTACTATAAAAAAGATTATAATAAAGGCATGATTAGTTACTAAAGAATTATAAATTTGATCATTATTAATTAATGAATTTGAAGAACCTAATTCTAATCGAATAATTATACTTATAGAAGATCCAATTATTCCAGCTCAGATAGCAAATAAAAAATAAAGAATTCCAATATCTTTATGTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formicidae was found to be postive for Wolbachia.
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