April 30, 2025

Sample information
Picture
Photos by: Clark T
Location
Collection date
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Freshwater stream in a forested area in the backyard of a house and scooped up from the stream with a net.
Putative identification Arthropoda Crustacea Malacostraca Decapoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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