Halictus ligatus

April 24, 2025

Sample information
Picture
Photos by: Nasir A.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students
Putative identification Arthropoda Insecta Hymenoptera Halictidae Halictus Halictus ligatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I had a thick Wolbachia gel band and a 100% ID match in BLAST.
Wolbachia 16S sequence Download FASTA    Download AB1
TTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGCCGAACCGACCCTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AAAAATGATGTATTTAAATTTCGATCTGTTAATAATATTGTAATAGCTCCTGCTAAAACTGGAAGAGAGAGAAGAAGTAAAATTGCAGTAATTTTTACTGATCAAGGAAATAGAGGGATTTGATTTATATTAAGTGAAATATTTTTTATTAAAATAATAGTTACAATAAAATTAATAGCTCCTATGATTGAAGAAATTCCTGCTATATGTAAGGAGAAGATAGAAAAATCTACTGAAAAAGATGAATGAAATATAATTGATGATAATGGAGGGTAAATTGTTCATCCTGTTCCTGAACCTGAAGTTAAAGTATTTCCTATTAATAGTAGTAATAAGGAAGGAATTAATAATCAAAATCTTATATTATTTATTCGTGGGAAAGCTATATCTGGGGCCCCAATTATTAAGGGTACTAATCAATTTCCAAATCCTCCAATTATGAATGGTATAACTATAAAGAAAATTATAATAAAAGCATGGGCAGTAACAATAGTATTATAAATTTGATCATTATTAATTCATCTTCCAGGAGTTGCTAGTTCTATACGGATAATTATACTTAGTGATGCACCAATTATTCCTGATCATATAGCAAAAATAAAGTAAAGTATACCAATATCTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Halictus ligatus was found to be postive for Wolbachia.
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