Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/15/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | I am confident that it is a Harmonia Axyridis but I was uncertain if I had Wolbachia because it did not pop up in the gel. However I did end up getting a Wolbachia DNA Sequence but it wasn’t strong enough to show up in the blast. |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid