NV 54 Fungus Gnat

April 15, 2021

Sample information
Picture
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

The sample was found alone in the mulch near flowers. The small, black insect has the ability to fly and walks at a slow pace.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes
  1. Arthropod Collection and Identification – The arthropod must be collected and placed in a closed container. Then it was identified using iNaturalist.
  2. DNA extraction – The arthropod was then crushed and a part of the fungus gnat was dissected.
  3. DNA amplification – The DNA was then amplified so it would be easier in the next step to visualize.
  4. DNA Visualization – A gel electrophoresis was run in order to visualize the results.
  5. DNA Sequence – To analyze the data from the gel electrophoresis and gain a better understanding of the DNA the nucleotides present are graphed.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We are highly confident because in both the lighter and darker filtered photos the gel did not show a band. Lane 54 has a smear to show the DNA was correctly collected but there is no band present.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Diptera was found to be negative for Wolbachia.
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