Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/04/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Neuqua Valley High School |
| Observations | I found it on my clothing in a pumpkin farm so it wasn’t in its natural habitat. But we know the location it came from. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
| Extraction kit | Puregene (Qiagen) |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | LB |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The gel electrophoresis did not show any bands in regular or high contrast. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Araneae was found to be negative for Wolbachia. |
Water Boatman