Goldenrod Soldier Beetle

April 25, 2025

Sample information
Picture
Photos by: Yara J
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Yellow beetle
Putative identification Arthropoda Hexapoda Insecta Coleoptera Cantharidae Chauliognathus Chauliognathus pensylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There was no wolbachia strand present on the gel electrophoresis
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
AnAnGnnnnnnTnAAnTTTCnATCTGTnnnnnnnnTnnnnnnnnnnCnnGnnnnAACAGGTAATGAAnnnnnAAnGAnnA nGGnnnnnATTCCTACTGCTCACnnnAnTAAGGGGGTTTGAnCnAnAnnnnTnCnnnnAGnTCGTATATTAATTnTnnnT GAAATAAAATTAATTGCCCCTAAAATnnAnGAnnTTnnnGnTnnGnGTAAnGAnnnnAnGGCTAAATCTACnGATGCTnC TCCGnGAnnnATTAAnGAnnAnAATGGAGGnnnnnnnGTTCAACCnnnTCCTGCTCCnTTTTCTnCTATTCTTCTAnAnn nnAnnnnnnnnnnnGAnGGnGGAAnAnnCCnAAnTCTnnnnTnGnTTATTCnGnnnnnnnGnnnTATCAGGnnnTnnnnn nnnnnnnGnnnnTAnnnnnTTTCnnAnnnnnnnnnTnnnAnTGGnnATAAnnATAAAnAAAATnATnnnnnATGnnnGnn nnGnTnCnnTGACnTnATAAATTTGAnnnnnnnnnATAnnnnnnnCTGGnnGnnnnnnTTCnnnTCnnnnnnnnnnnCTn AnAnA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Chauliognathus pensylvanicus was found to be negative for Wolbachia.
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